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lipofectin transfection method  (Thermo Fisher)


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    Thermo Fisher lipofectin transfection method
    Lipofectin Transfection Method, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipofectin+transfection+method/pm17512906-48-17-20
    Average 86 stars, based on 1 article reviews
    lipofectin transfection method - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Transfection:

    Article Title: Akt protein kinase inhibits Rac1-GTP binding through phosphorylation at serine 71 of Rac1.
    Article Snippet: Rac1 and Rac1 S71A Expression Vector Transfection and Purification—Hexahistidine-tagged Rac1, Rac1 S71A, and dominant-positive Akt kinase were each cloned in the pUSEamp(1) mammalian expression vector (Upstate Biotechnology, Inc.). .. They were transfected or cotransfected in SK-MEL28 cells following the Lipofectin transfection method (Life Technologies, Inc.). ..

    Article Title: Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins.
    Article Snippet: The Aspergillus nidulans protein NIMA (never in mitosis, gene A) is a protein kinase required for initiation of mitosis, whereas its inactivation is necessary for mitotic exit.. Here, we present evidence that human Nek6 is associated with Fe65.. Based on the presence of Fe65 WW domain binding motifs (PPLP) in the Nek6 catalytic domain, we observed that Nek6 interacts physically with Fe65 both in vivo and in vitro, using a pull-down approach.

    Expressing:

    Article Title: Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins.
    Article Snippet: The Aspergillus nidulans protein NIMA (never in mitosis, gene A) is a protein kinase required for initiation of mitosis, whereas its inactivation is necessary for mitotic exit.. Here, we present evidence that human Nek6 is associated with Fe65.. Based on the presence of Fe65 WW domain binding motifs (PPLP) in the Nek6 catalytic domain, we observed that Nek6 interacts physically with Fe65 both in vivo and in vitro, using a pull-down approach.

    Mutagenesis:

    Article Title: Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins.
    Article Snippet: The Aspergillus nidulans protein NIMA (never in mitosis, gene A) is a protein kinase required for initiation of mitosis, whereas its inactivation is necessary for mitotic exit.. Here, we present evidence that human Nek6 is associated with Fe65.. Based on the presence of Fe65 WW domain binding motifs (PPLP) in the Nek6 catalytic domain, we observed that Nek6 interacts physically with Fe65 both in vivo and in vitro, using a pull-down approach.

    Construct:

    Article Title: Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins.
    Article Snippet: The Aspergillus nidulans protein NIMA (never in mitosis, gene A) is a protein kinase required for initiation of mitosis, whereas its inactivation is necessary for mitotic exit.. Here, we present evidence that human Nek6 is associated with Fe65.. Based on the presence of Fe65 WW domain binding motifs (PPLP) in the Nek6 catalytic domain, we observed that Nek6 interacts physically with Fe65 both in vivo and in vitro, using a pull-down approach.

    other:

    Article Title: Arsenite inhibits interleukin-6 production in human intestinal epithelial cells by down-regulating nuclear factor-kappaB activity.
    Article Snippet: Previous studies have suggested that the production of interleukin-6 (IL-6) is increased in the intestinal mucosa during inflammation, and that nuclear factor-κB (NF-κB) is an important regulator of the IL-6 gene in the enterocyte.. We tested the hypothesis that sodium arsenite inhibits IL-6 production in stimulated enterocytes and that this effect of arsenite is caused by down-regulation of NF-κB activity.. Cultured Caco-2 cells were treated with sodium arsenite and were then stimulated with IL-1β.



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    FIG. 7. The IE-TFIIB interaction domain(s) is physiologically significant for the transactivating ability of the IE protein. Transient-cotrans- fection assays assessing transactivation of the representative EHV-1 early TK and late IR5 promoters linked to the CAT reporter gene by effector constructs expressing the IE deletion mutants targeting the TFIIB-binding domain were performed as described in Materials and Methods. All <t>transfections</t> were performed in triplicate. The error bars show standard deviations. (A) Transactivation of the viral pTK-CAT (early) reporter construct (1.4 pmol). (B) Transactivation of the viral pIR5-CAT (late) reporter construct (2.0 pmol).
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    FIG. 7. The IE-TFIIB interaction domain(s) is physiologically significant for the transactivating ability of the IE protein. Transient-cotrans- fection assays assessing transactivation of the representative EHV-1 early TK and late IR5 promoters linked to the CAT reporter gene by effector constructs expressing the IE deletion mutants targeting the TFIIB-binding domain were performed as described in Materials and Methods. All transfections were performed in triplicate. The error bars show standard deviations. (A) Transactivation of the viral pTK-CAT (early) reporter construct (1.4 pmol). (B) Transactivation of the viral pIR5-CAT (late) reporter construct (2.0 pmol).

    Journal: Journal of Virology

    Article Title: Mapping the Sequences That Mediate Interaction of the Equine Herpesvirus 1 Immediate-Early Protein and Human TFIIB

    doi: 10.1128/jvi.75.21.10219-10230.2001

    Figure Lengend Snippet: FIG. 7. The IE-TFIIB interaction domain(s) is physiologically significant for the transactivating ability of the IE protein. Transient-cotrans- fection assays assessing transactivation of the representative EHV-1 early TK and late IR5 promoters linked to the CAT reporter gene by effector constructs expressing the IE deletion mutants targeting the TFIIB-binding domain were performed as described in Materials and Methods. All transfections were performed in triplicate. The error bars show standard deviations. (A) Transactivation of the viral pTK-CAT (early) reporter construct (1.4 pmol). (B) Transactivation of the viral pIR5-CAT (late) reporter construct (2.0 pmol).

    Article Snippet: L-M cells (3 106) were plated in tissue culture dishes (60-mm diameter) at approximately 75% confluence and transfected with plasmid DNAs by the Lipofectin-mediated DNA transfection method (Gibco BRL) (32, 55).

    Techniques: Construct, Expressing, Binding Assay, Transfection